Format: Biotin; Reactivity: human, rat, dog, bovine, monkey, hamster, chicken
Articlecode: 03-61513
Product: Anti-Vimentin
Catalog #: 03-61513 Biotin
Amount: 50 ml
CATEGORY: Mouse monoclonal
CLONE DETERMINATION: VIM3B4
IMMUNOGLOBULIN CLASS: IgG2a
IMMUNOGEN: Vimentin, purified from bovine lens
ANTIGEN RECOGNIZED IN SPECIES: human, monkey, bovine, hamster, rat, dog, chicken, amphibian, weaker murine cross reaction
PURIFICATION: SpAss Protein A (recombinant)
APPLICATION: Immunohistochemistry, suitable for frozen and paraffin embedded tissue (protease pretreatment required)
Cytological material
Immunofluorescence
Immunoblotting (Western)
ELISA
POLYPEPTIDE REACTING: MR 57,000 intermediate filament protein (vimentin) of Mesenchymal cells vesicles.
REACTIVIES ON CULTURED LINES: RD cells, glioma cells, fibroblasts (SV-80, 3T3), MDCK
TUMORS SPECIFICALLY DETECTED: Sarcoma (including myosarcoma), lymphoma, melanoma
WORKING DILUTION: Dilute further to at least 1:100 for immunohistochemistry
Prediluted: Ready to Use for immunohistochemistry
INCUBATION TIME: 1 hour at room temperature, extended with paraffin (overnight at 4°C)
STORAGE: 2-8°C for immediate use, or at -20°C (aliquot)
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Kasper, M.et al. (1989). Histochemistry 93: 93-103
Mol, I. & Moll, R. (1991) Differentiation 40: 207-214
Rungger-Braendle, E. et al. (1989) J Cell Biol. 109: 705-716
Gomi, H. et al. (1995) Neuron 14: 29-41
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Hermann, H. et al. (1993) J. Mol. Biol 234: 99-113
Rogers, KR et al. (1995) Eur J Cell Biol 66: 136-150
Bohn, W. et al. (1992) Exp. Cell Res 201: 1-7
Hermann, H. et al. (1992) J. Mol Biol 223: 637-650
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