Format: Supernatant; Reactivity: Xenopus, Trout, Carp
Articlecode: 03-65189
Product: Anti-Vimentin
Catalog #: 03-65189
Amount: 1 ml
CATEGORY: Mouse monoclonal
CLONE DETERMINATION: XL-VIM-14.13
IMMUNOGLOBULIN CLASS: IgG1
IMMUNOGEN: Vimentin from cytoskeletal fraction of XLKE cells (cultured Xenopus laevis kidney epithelial cells)
FORM: Hybridoma culture supernatant
ANTIGEN RECOGNIZED IN SPECIES (tested so far): Xenopus laevis, rainbow trout (Onchorynchus mykiss), carp; human and bovine are negative
APPLICATION: Immunohistochemistry, suitable for frozen sections
Immunoblotting (Western)
POLYPEPTIDE REACTING: MR 53,325 (pI 4.95) intermediate filament protein (vimentin) of Xenopus laevis (epitope presumably located between amino acids 79 and 88 within rod domain).
CLINICAL APPLICATION/SPECIFICITY: Vimentin of amphibia and fish, predominantly found in glial and white blood cells
WORKING DILUTION: Ready to use for immunohistochemitry
INCUBATION TIME: 1 hour at room temperature, extended with paraffin (overnight at 4°C)
STORAGE: 2-8°C for immediate use, or at -20°C (aliquot)
REFERENCES:
Herrmann, H. et al. (1996) J. Cell Sc 109: 569-578
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