Sandwich ELISA kits for measurement of cytokines in serum or other biological samples. All reagent are supplied for 96 single well assays. Each component has been pretested according to protocol, so all reagents are optimized for ELISA. Prestained TMB (pink-ONE) has advantages as follows: No mixing of TMB substrate and substrate solution required. Pink-color coded indicator : Convenient to track for reagent supply. No interference of color to activity or sensitivity of reaction (H2SO4 makes color disappeared).

Antigen-affinity purified antibody Pre-Coated 96 well ELISA microplate Biotinylated antigen-affinity purified detection antibody Standard Protein (Lyophilized) : Recombinant protein Color development Enzyme (Lyophilized) : Avidin-HRP conjugate Assay Diluting Solution : 0.1% BSA in PBST (50 ml) pink-ONE TMB Substrate Stop Solution : 2M H2SO4 (10 ml) PBS powder Tween-20 (50%)