Reactivity: Human
Articlecode: 03-62052
Product: sm RNP (B'B & D Proteins)
Catalog #: 03-62052
Amount: 20 mg
SOURCE: HeLa Cells (human cell line)
MOLECULAR WEIGHT: 28,000 (B); 28,500 (B''); 29,000 (B') (SDS-PAGE)
ISOELECTRIC POINT: pHI: 10
APPLICATION: Reference protein in SDS-PAGE and immuno blotting (Western blotting) or dot blotting
1-2 µg protein per lane is sufficient for SDS-PAGE
0.5 µg protein sufficient for dot or immunoblotting
Coating protein for immunoassays
50 ng-100ng protein per well is sufficient
The B,B'- proteins are targets for anti-Sm sera.
Autoimmune antibodies against these proteins are present in sera from patients with SLE and MCTD.
Some anti-RNP sera show also a reactivity against B,B' proteins, but normally with weak titer.
Some antibodies against the B'' protein show cross-reactivity with the A protein.
RECONSTITUTION: When reconstituted with 200µl H20 bidest, solution will contain 25mM Tris, 190mM Glycin, pH 8.3, 0.025 % (w/v) SDS with a protein conc. of 0.1 mg/ml (according to Bradford protein determination). For SDS-PAGE and immunoblotting reconstitution with 200 µl Laemmli buffer is recommended.
PURITY & IDENTITY: Preparation shows one double band at 28 kDa in SDS-PAGE analysis. These bands are specifically immunoreactive with an anti Sm mAb (H126 cat. #03-61052)
STORAGE: 2-8°C for immediate use, or at -20°C (aliquot)
REFERENCES:
Combe, B.et al. (1989) Clin. exp. Immunol. 75: 18 ff
Guldner, H.H.et al. (1986) Clin. Immunol. Immunopathol. 40: 532 ff
Habets, W.J.et al. (1990) Arthritis Rheum. 33 (6): 834 ff
Lührmann, R. (1988): In: Structure and function of major and minor small
nuclear ribonucleoprotein part icles. Birmstil, M.L. (ed.), Springer Verlag, Berlin/New York, pp. 71 ff
Reichlin, M. and Van Venrooij, W.J (1991) Clin. exp. Immunol. 83: 286 ff
Takeda, Y. et al. (1989) Clin. Immunol. Immunopathol. 50: 213 ff
Tan, E.M (1989) Adv. Immunol. 44: 93 ff
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