14-3-3 α/β polyclonal, anti-human, mouse, rat

Background:
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Alternative Name:
KCIP-1, YWHAB, 14 3 3 alpha, 14 3 3 protein beta/alpha, 14-3-3 protein beta/alpha, 1433B, Brain protein 14 3 3 beta isoform, GW128, HS 1, KCIP-1, KCIP1, N-terminally processed, Protein 1054, Protein kinase C inhibitor protein 1, YWHAA, YWHAB,

Application Dilution:
WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Specificity: 14-3-3 α/β polyclonal antibody detects endogenous levels of 14-3-3 α/β protein.

Immunogen:
recombinant protein

MW: 28 kDa

Swiss-Prot: P31946(Human) Q9CQV8(Mouse) P35213(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.