4E-BP1 polyclonal, anti-human, mouse, rat

Background:
The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E.

Alternative Name:
4E-BP1, eIF4E-binding protein 1, PHAS-I, EIF4EBP1, 4E-BP1, 4EBP1, 4EBP1, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eif4ebp1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1,

Application Dilution:
WB:1:500-1:1,000
ICC:1:500-1:1,000
IHC:1:50-1:200
FC:1:50-1:100

Specificity: 4E-BP1 polyclonal antibody detects endogenous levels of 4E-BP1 protein.

Immunogen:
Recombinant protein.

MW: 12.5 kDa

Swiss-Prot: Q13541(Human) Q60876(Mouse) Q62622(Rat)

Purification & Purity:
Protein affinity purified.

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.