53BP1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
TP53BP1(Tumor Protein p53-Binding Protein 1), also called 53BP1, is a protein that in humans is encoded by the TP53BP1 gene. Iwabuchi et al.(1998) mapped the TP53BP1 gene to 15q15-q21 by FISH. Iwabuchi et al.(1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro. Immunoblot analysis by Iwabuchi et al.(1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21(CDKN1A). Wang et al.(2002) used small interfering RNA directed against TP53BP1 in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal. TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.

Description:
Rabbit IgG polyclonal antibody for Tumor suppressor p53-binding protein 1 (TP53BP1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.

Synonyms:
53 BP1 antibody, 53BP1 antibody, FLJ41424 antibody, MGC138366 antibody, p202 antibody, p53 binding protein 1 antibody, p53 BP1 antibody, p53-binding protein 1 antibody, p53BP1 antibody, TP53 BP1 antibody, TP53B_HUMAN antibody, TP53BP1 antibody, TRP53 BP1 antibody, Tumor protein 53 binding protein 1 antibody, Tumor protein p53 binding protein 1 antibody, Tumor suppressor p53 binding protein 1 antibody, Tumor suppressor p53-binding protein 1 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1(1955-1972aa ERIGFKQHPKYKHDYVSH), identical to the related mouse sequence.A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1(1955-1972aa ERIGFKQHPKYKHDYVSH), identical to the related mouse sequence.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.