6×His polyclonal, anti-fusion proteins containing the HIS Tag

Background:
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.

Synonyms: 6×His

Immunogen:
A synthetic peptide

Gene Symbol: 6×His

Calculated MW: 49kDa/45 kDa

Recommended dilution:
WB 1:3000-7000

Purity:
Affinity purification

Storage Buffer:
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.

Storage:
Store at -20℃. Avoid freeze / thaw cycles.