6-keto-PGF1a Detection Kit

Use: Used to detect the concentration of 6-keto-PGF1a in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled 6-keto-PGF1a and purified anti-6-keto-PGF1a antibody are coated on the microplate. In the competitive inhibition reaction, a certain amount of solid phase antibody and biotin-labeled 6-keto-PGF1a and non-labeled antigen (calibrator or specimen) are used to inhibit the competitive reaction. a) The binding amount is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less the antibody binds to the biotin-labeled 6-keto-PGF1a, and vice versa. After the reaction is balanced, a solid-phase antibody-biotinylated 6-keto-PGF1a is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated 6-keto-PGF1a-enzyme-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of 6-keto-PGF1a increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation, and rapidity, and has reliable detection performance for the reduction or increase of 6-keto-PGF1a in the sample.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.