Acidic Hair Keratin K32 (hHa2) polyclonal, anti-human, negative for mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription.

Synonyms:
KRT32, HA2, HKA2, hHa2, KRTHA2

Isotype: Guinea pig Polyclonal (GP-hHa2)

Immunogen:
Synthetic peptide of human hair (trichocytic) keratin K32 (formerly also designated keratin hHa2; C- VTRTVCVPRTVGM), coupled to KLH

Form: Stabilized Serum

Specificity:
The antiserum stains specifically human hair keratin K32 expressed in the hair cuticle (Mr 45,000 polypeptide) and tumors derived from.

Reactivity: human, negative for mouse

Applications: Immunohistochemistry on frozen sections and paraffin embedded material after trypsin and microwave pretreatment), Immunoblotting (Western)

Working DIlution: 1:200 for immunohistochemistry; 1:2000 for Immunoblotting

Storage: 2-8C

References:
- Langbein L. et al. (1999). J Biol Chem 274: 19874 – 19893
- Langbein L. (2004) Methods in Cell Biology 78: 413-456
- Langbein L, Schweizer J (2005) Intern Rev Cytology 243: 1-78