Acidic Hair Keratin K35 (hHa5) polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription.

Synonyms:
KRT35, HA5, HKA5, hHa5, KRTHA5

Isotype: Guinea pig Polyclonal (GPhHa5)

Immunogen:
Synthetic peptide of human hair (trichocytic) keratin K35, (formerly also designated hHa5, YSS SPCKLP SLS PVA RS), conjugated to KLH

Form: Stabilized Serum

Specificity:
The antiserum stains specifically human hair keratin K35 expressed starting from the upper part of hair matrix, lower cortex, and hair cuticle.

Reactivity: human, mouse (reduced cross-reaction)

Applications: Immunohistochemistry on frozen sections, Immunoblotting

Working DIlution: 1:200 for immunohistochemistry; 1:2,000 for immunoblotting (ECL method)

Storage: 2-8C

References:
- Langbein, L. et al. (1999) J. Biol. Chem. 274: 19874-19893
- Langbein L et al. (2004) Methods in Cell V Biology 78: 413-456