Actin alpha-skeletal (clone 3B3), anti-human, rat, caprine, rabbit, swine
€564.00
In stock
SKU
ARP-21-5002
Catalog Number: 21-5002
Size: 100 µg
Isotype: Mouse IgG1
Size: 100 µg
Isotype: Mouse IgG1
Background:
Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebRates. Skeletal alpha-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development alpha-cardiac actin becomes the main isoform in the myocardium, alpha-skeletal actin might still make up for almost half of its total striated muscle actin content.
Clone: 3B3
Isotype: Mouse IgG1
Source: 3B3 is a Mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
Reactivity: caprine, human, rabbit, rat, swine. The epitope recognized by alpha-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
Specificity: Clone 3B3 is an antibody highly specific for alpha-skeletal actin, and does not cross react with other actin isoforms.
Form: Each vial contains 100ul purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applications: ELISA, Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Western blotting
Storage: Store at 4C, or in small aliquots at -20C.
Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebRates. Skeletal alpha-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development alpha-cardiac actin becomes the main isoform in the myocardium, alpha-skeletal actin might still make up for almost half of its total striated muscle actin content.
Clone: 3B3
Isotype: Mouse IgG1
Source: 3B3 is a Mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
Reactivity: caprine, human, rabbit, rat, swine. The epitope recognized by alpha-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
Specificity: Clone 3B3 is an antibody highly specific for alpha-skeletal actin, and does not cross react with other actin isoforms.
Form: Each vial contains 100ul purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applications: ELISA, Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Western blotting
Storage: Store at 4C, or in small aliquots at -20C.
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