ACTN1 Polyclonal Antibody

Background:
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

Research Area: Cancer, Developmental Biology, Signal Transduction, Stem Cells

Synonyms:
ACTN1, BDPLT15

Immunogen: Recombinant fusion protein of human ACTN1 (NP_001123477.1).

Swissprot: P12814
Gene ID: 87

Calculated MW: 102 kDa/103 kDa/105 kDa/107 kDa
Observed MW: 105 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IHC 1:50-1:200"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.