ADAR polyclonal, anti-human

Background:
RNA-specific adenosine deaminase (ADAR1, DSH, IFI4, p136, DRADA, DSRAD, K88dsRBP) mediates RNA editing by destabilizing double stranded RNA through deamination of adenosine to inosine in structured or double-stranded RNAs. ADAR1 is expressed from an interferon-response promoter and has a Z-DNA/Z-RNA binding domain at its N-terminus. ADAR1 co-localizes with SUMO-1 in a subnucleolar region that is distinct from the fibrillar center, the dense fibrillar component and the granular component. Localization of nuclear ADAR1 is under the influence of a nucleolar localization signal (NoLS) in the middle of ADAR1 and the exporting activity of the nuclear exporter signal (NES) near the N terminus. ADAR1 upregulates nuclear factor 90 (NF90)-mediated gene expression by interacting with NF110, NF90 and NF45. ADAR1 binds short interfering RNA (siRNA), and gene silencing by siRNA is significantly more effective in mouse fibroblasts homozygous for an ADAR1 null mutation than in wild-type cells. ADAR1 may limit the efficacy of siRNA in mammalian cells.

Alternative Name:
DRADA, p136, IFI-4, ADAR, ADAR1, DSRAD, G1P1, IFI4, 136 kDa double-stranded RNA-binding protein, 136kDa double stranded RNA binding protein, Adar 1, ADAR, Adar1, Adenosine deaminase acting on RNA 1 A, Adenosine deaminase RNA specific 1, Adenosine deaminase RNA specific, Adenosine deaminase that act on RNA, AGS6, AV242451, Double stranded RNA specific adenosine deaminase, Double-stranded RNA-specific adenosine deaminase, Double-stranded RNA-specific editase Adar, DRADA, Dsh, Dsrad, DSRAD, dsRNA adenosine deaminase, EC 3.5.4.-, G1P1, IFI 4, IFI-4, IFI4, Ifi4 protein, Interferon induced protein 4, Interferon inducible protein 4, Interferon-inducible protein 4, K88DSRBP, mZaADAR, P136, Pre-mRNA adenosine deaminase, RNA adenosine deaminase 1, RNA-editing deaminase 1, RNA-editing enzyme 1,

Application Dilution:
WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: ADAR polyclonal antibody detects endogenous levels of ADAR protein.

Immunogen:
Recombinant protein

MW: 136 kDa

Swiss-Prot: P55265(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.