Adiponectin (clone MADI 1147), anti-mouse

Decription:
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.

Synonyms: ACRP30, AdipoQ, apM1, GBP28, Adipocyte Complement Related Protein of 30kDa

Clone: MADI 1147

Isotype: Mouse IgG1 kappa

Immunogen: Recombinant mouse adiponectin.

Reactivity: mouse

Specificity: Recognizes mouse adiponectin. Detects a band of ~30kDa by Western blot. Weakly cross-reacts with human and rat adiponectin.

Form: Purified, in 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Applications:
ELISA: (direct and indirect: 1:5'000-1:10'000).
Immunoprecipitation: (1-5?g per sample in a final reaction volume of 500-1000?l). Western blot: (1:5'000-1:10'000 using ECL; suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature).

Working Dilution: Optimal conditions must be determined individually for each application.

Concentration: 1mg/ml

Storage: 2-8C immediate use, or -20C (aliquote)