Adiponectin monoclonal, anti-human

Source:
Produced in Balb/c mice using highly pure recombinant Human Adiponectin as the immunizing antigen. This antibody was purified from cell culture by Protein A affinity chromatography.

Western Blot:
To detect Human Adiponectin by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Adiponectin is 0.5-1.0 ng/lane, under reducing conditions and 1.0-2.0 ng/lane, under non-reducing conditions.

Sandwich ELISA:
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant Human Adiponectin when used in conjunction with compatible detection reagents at a concentration of approximately 0.5-1.0 μg/ml.

Formulation: A sterile filtered antibody solution was lyophilized from PBS.

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.