Adipophilin, C-terminal, (ADRP) polyclonal, anti-human

Quantity: 25 µl

Background:
The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.

Synonyms:
ADFP, ADRP

Isotype: Guinea pig Polyclonal (GP41 hCTA/B)

Immunogen:
Synthetic peptide (C-terminal aa 423 - 437 of human adipophilin)

Form: Stabilized Serum

Specificity:
Adipophilin is positively detected in the glandular cells of lactating mammary gland ( ductal cells are negative), zona fasciculate of the adrenal gland, Sartoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Also positive stained are lipid-storing CD68-positive macrophages.

Reactivity: human

Applications: Immunofhistochemistry on frozen (see Ohsaki et al.) and paraffin tissues after microwave treatment, Immunoblotting (western, ECL), Cytological material

Working DIlution: 1:100 for Immunofhistochemistry applications; 1:2000 for Immunoblotting (western, ECL)

Positive Control:
PLC, SV80

Storage: 2-8C

References:
- Heid, HW. et al. (1998) Cell Tissue Res 294: 309-321Heid, HW. et al. (1996) Biochem J. 320: 1025-1030
- Mrozinzki, J. et al. (1999) J. Cancer Res. & Clin. Oncol. 125: suppl.
- Steiner, S. et al. (1996) Biochem, Biophys Res. 218: 777-782
- Hope, RG & MaLauchlan, J. (2000) J. Gen. Virol. 81: 1913-1925
- Imamura, M. et al. (2002) Am J Physiol Endocrinol Metab 283: E775-E783
- Targett-Adams, P. et al. (2003) J Biol Chem, Manuscript M211289200
- Imai Y et al. (2007) Gastroenterology 132: 1947-1954
- Ohsaki, Y. et al. (2005) Histochem Cell Biol 124: 445-452
- Samsa MM et al. (2009) PLOS Pathogens 5(10) e1000632