Adipophilin, N-terminal, (ADRP) polyclonal, anti-human, bovine, mouse, rat, canine

Quantity: 25 µl

Background:
The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.

Synonyms:
ADFP, ADRP

Isotype: Guinea pig Polyclonal (GP40, mN1)

Immunogen:
Synthetic peptide (N-terminal aa 1-29 of human and murine adipophilin)

Form: Stabilized Serum

Specificity:
Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculate of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Specific detection of liposarcoma. Also positively stained are lipid-storing CD68-positive macrophages.

Reactivity: human, bovine, mouse, rat, Canine

Applications: Immunohistochemistry on frozen and paraffin sections after microwave treatment, Immunoblotting, Cytological Material

Working DIlution: 1:100 for immunohistochemistry; 1:2,000 for immunoblotting (ECL method)

Positive Control:
Caco, PLC, HaCat, SV80, RD125, Huvec (human umbilical cord endothelia), RV, PC-12 (rat adrenal gland), MDCK; negative with glioma

Storage: 2-8C

References:
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- Heid, HW. et al. (1996) Biochem J. 320: 1025-1030
- Mrozinzki, J. et al. (1999) J. Cancer Res. & Clin. Oncol. 125: suppl.
- Steiner, S. et al. (1996) Biochem, Biophys Res. 218: 777-782
- Hope, RG & MaLauchlan, J. (2000) J. Gen. Virol. 81: 1913-1925
- Imamura, M. et al. (2002) Am J Physiol Endocrinol Metab 283: E775-E783
- Targett-Adams, P. et al. (2003) J Biol Chem, Manuscript M211289200
- Imai Y et al. (2007) Gastroenterology 132: 1947-1954
- Ohsaki, Y. et al. (2005) Histochem Cell Biol 124: 445-452
- Samsa MM et al. (2009) PLOS Pathogens 5(10) e1000632