Adrenomedullin 1-6 polyclonal, anti-rat

Quantity: 50 µl

Background:
Adrenomedullin, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. Genomic ADM DNA consists of 4 exons and 3 introns, with the 5-prime flanking region containing TATA, CAAT, and GC boxes. There are also multiple binding sites for activator protein-2 and a cAMP-regulated enhancer element.

Synonyms:
ADM, AM

Isotype: Guinea Pig Polyclonal

Immunogen:
Synthetic rat adrenomedullin 1-6, conjugated to BSA

Form: Serum containing 0.1% sodium azide

Specificity:
The antiserum was raised using synthetic peptides as immunogens. The antibody does not cross-react with CGRP.

Reactivity: rat

Applications: Immunofluorescence, Immunohistochemistry, suitable for frozen sections

Working DIlution: 1:40 to 1:80 immunofluorescence microscopy with FITC

Positive Control:
Stefanini-fixed frozen sections of rat fundus

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
- Mulder, H. et al. (1996) Regulatory Peptide 62: 107-112