AFP (Alpha Fetoprotein) (clone C3), anti-human, monkey, dog, and pig

AFP (Alpha Fetoprotein) (clone C3), anti-human, monkey, dog, and pig

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In stock
SKU
ARP-24-174-MSM1
Catalog Number: 24-174-MSM1
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG2a, kappa
Decription:
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This monoclonal antibody is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Synonyms: Alpha fetoglobulin, FETA, HPAFP

Clone: C3

Isotype: Mouse IgG2a, kappa

Immunogen: Alpha fetoprotein (AFP) purified from serum of a hepatoma patient

Reactivity: human, monkey, dog, and pig. It does not react with cow, dog, mouse, and rat.

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Reactivity: Hep-G2 cells. Fetal liver or hepatocellular carcinoma

Concentration: 0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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