AIF (clone 8H1-B10-A12), anti-human

Background:
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10.

Synonyms: AIF, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, NADMR, NAMSD, PDCD8

Cellular Location: Cytoplasm Mitochondrion inner membrane Mitochondrion intermembrane space Nucleus perinuclear region

Immunogen:
Purified recombinant human AIF protein fragments expressed in E.coli.

Gene Symbol: AIF

Gene ID: 9131

Swiss prot: O95831

Calculated MW: 67kDa

Recommended dilution:
WB 1:1000, ICC 1:200

Purity:
Affinity purification

Storage Buffer:
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:
Store at -20℃. Avoid freeze / thaw cycles.