Akt (Ser-473), phospho-specific (clone M114), anti-human, mouse, rat
€410.00
In stock
SKU
ECM-AM1141
Catalog Number: ECM-AM1141
Size: 200 μl
Isotype: mouse IgG1
Applications: WB, E, IP
Reactivity: Hu, Ms, Rt
Datasheet
Questions? Contact us!
Size: 200 μl
Isotype: mouse IgG1
Applications: WB, E, IP
Reactivity: Hu, Ms, Rt
Datasheet
Questions? Contact us!
Background:
Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.
Background References
Jones, P.F. et al. (1991) Proc Natl Acad Sci. USA 88:4171.
Marte, B. & Downward. J. (1997) TIBS. 22:355-358.
Powell, D.J. et al. (2003) Mol. Cell Biol. 23:7794-7808.
Immunogen: Clone M114 was generated from a peptide containing amino acid residues surrounding Serine 473 in human Akt1. This sequence is highly conserved in human and mouse Akt, and may recognize Akt2 and Akt3.
Specificity: This antibody detects a 60 kDa* protein corresponding to the apparent molecular mass of Akt on SDS-PAGE immunoblots of A431 cell lysate. Similar results were seen in various other human, rat, and mouse lysates.
Application dilution:
ELISA: 1:2000
IP: 1:100
WB: 1:250
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Product References:
Wu, C. et al. (2012) Cell. 148(5):973. WB: mouse embryonic fibroblasts
Paterniti, I. et al. (2011) Eur J Neurosci. 33(8):411. WB: mouse spinal cord
Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.
Background References
Jones, P.F. et al. (1991) Proc Natl Acad Sci. USA 88:4171.
Marte, B. & Downward. J. (1997) TIBS. 22:355-358.
Powell, D.J. et al. (2003) Mol. Cell Biol. 23:7794-7808.
Immunogen: Clone M114 was generated from a peptide containing amino acid residues surrounding Serine 473 in human Akt1. This sequence is highly conserved in human and mouse Akt, and may recognize Akt2 and Akt3.
Specificity: This antibody detects a 60 kDa* protein corresponding to the apparent molecular mass of Akt on SDS-PAGE immunoblots of A431 cell lysate. Similar results were seen in various other human, rat, and mouse lysates.
Application dilution:
ELISA: 1:2000
IP: 1:100
WB: 1:250
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Product References:
Wu, C. et al. (2012) Cell. 148(5):973. WB: mouse embryonic fibroblasts
Paterniti, I. et al. (2011) Eur J Neurosci. 33(8):411. WB: mouse spinal cord
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