AKT1/2 polyclonal, anti-human

Quantity: 100 µg

Background:
AKT protein family, which members are also called protein kinases B(PKB) plays an important role in mammalian cellular signaling. In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3. These genes code for enzymes that are members of the serine/threonine-specific protein kinase family. Akt1 is involved in cellular survival pathways, by inhibiting apoptotic processes. Akt1 is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. Since it can block apoptosis, and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer. Akt(now also called Akt1) was originally identified as the oncogene in the transforming retrovirus, AKT8. AKT8 was isolated by Stephen Staal in the laboratory of Wallace P. Rowe; he subsequently cloned v-akt and human AKT1 and AKT2 while on staff at the Johns Hopkins Oncology Center. Akt2 is an important signaling molecule in the Insulin signaling pathway, it is required to induce glucose transport. Franke et al.(1995) show that AKT1 and AKT2 are activated by PDGF. The activation was rapid and specific, and it was abrogated by mutations in the Akt Pleckstrin homology(PH) domain. They identify that Akt is a novel target of PI 3-kinase and suggest that the Akt PH domain may be a mediator of PI 3-kinase signaling.

Description:
Rabbit IgG polyclonal antibody for RAC-alpha serine/threonine-protein kinase (AKT1) detection. Tested with WB in Human.

Synonyms:
AKT 1 antibody, AKT 2 antibody, AKT antibody, AKT1 antibody, AKT1 kinase antibody, AKT2 antibody, AKT2 kinase antibody, C AKT antibody, MGC99656 antibody, Murine thymoma viral(v akt) homolog 2 antibody, Oncogene AKT1 antibody, PKB alpha antibody, PKB antibody, PKB beta antibody, PKBB antibody, PKBBETA antibody, PRKBA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein Kinase B Alpha antibody, Protein kinase B antibody, Protein kinase B beta antibody, RAC antibody, RAC alpha antibody, RAC alpha serine/threonine protein kinase antibody, RAC BETA antibody, RAC beta serine threonine protein kinase antibody, RAC PK alpha antibody, RAC PK beta antibody, Rac protein kinase alpha antibody, Rac protein kinase beta antibody, RACbeta antibody, v akt murine thymoma viral oncogene homolog 1 antibody, v akt murine thymoma viral oncogene homolog 2 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human AKT1/2(70-90aa NTFIIRCLQWTTVIERTFHVE), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.