Albumin polyclonal, anti-rat

Quantity: 1 ml

Background:
The defined antibody specificity is directed to albumin as tested against rat sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified albumin. The cytochemical grade of GARa/Alb/7S allows its use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting.This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.Working dilutions for histochemical and cytochemical use are usually between 1:10 and 1:50; in ELISA and comparable non-precipitating antibody-binding assays are between 1:100 and 1:1,000.

Isotype: Goat Polyclonal

Source: Highly purified albumin isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Purified hyperimmune goat IgG, in phosphate buffered saline (PBS, pH 7.2)

Specificity:
Purified IgG fraction of polyclonal Goat anti-Serum to Rat albumin

Reactivity: rat

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results:cow - Goat - Human + Rabbit ± Chicken - Guinea Pig ++ Monkey + Sheep - Dog + Horse + Mouse ++ Swine +

Applications: Indirect immunofluorescence, ELISA, Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.