Albumin polyclonal HRP polyclonal, anti-human

Quantity: 200 µl

Background:
Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal human serum and purified human albumin. One characteristic precipitin line is obtained against pooled normal human serum using different antigen/antibody concentration ratio's. Precipitin lines against normal human serum and purified human albumin give a reaction of full identity. In enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure albumin in human serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:10,000.

Isotype: Goat Polyclonal

Source: Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from human serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Peroxidase-coupled purified hyperimmune goat IgG, in phosphate buffered saline (PBS, pH 7.2).

Specificity:
Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat anti-Serum to Human albumin

Reactivity: human

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.