Aldosterone (ALD) Detection Kit

Use: Used to detect the concentration of aldosterone (ALD) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled aldosterone (ALD) is used, and the purified anti-aldosterone (ALD) antibody is coated on the microplate. In the competitive inhibition reaction, a certain amount of solid-phase antibody inhibits the competitive reaction with biotin-labeled aldosterone (ALD) and non-labeled antigen (calibrator or specimen). The binding amount of antibody and biotin-labeled aldosterone (ALD) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of antibody and biotin-labeled aldosterone (ALD), and vice versa. After the reaction is balanced, solid-phase antibody-biotinylated aldosterone (ALD) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated aldosterone (ALD)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of aldosterone (ALD) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the decrease or increase of aldosterone (ALD) in samples.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.