ALIX / PDCD6IP Polyclonal Antibody

Background:
This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15.

Research Area: Cancer, Cell Biology, Microbiology, Signal Transduction

Synonyms:
PDCD6IP, AIP1, ALIX, DRIP4, HP95

Immunogen: Recombinant fusion protein of human ALIX / PDCD6IP (NP_037506.2).

Swissprot: Q8WUM4
Gene ID: 10015

Calculated MW: 30 kDa/96 kDa
Observed MW: 105 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:200-1:2000 IHC 1:50-1:200 IF 1:50-1:200"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.