Alkaline Phosphatase polyclonal, anti-human

Quantity: 100 µg

Background:
Alkaline phosphatase(ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific(found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Description:
Rabbit IgG polyclonal antibody for Alkaline phosphatase, tissue-nonspecific isozyme (ALPL) detection. Tested with WB in Human.

Synonyms:
Akp2 antibody, Alkaline phosphatase intestinal antibody, Alkaline phosphatase intestinal precursor antibody, Alkaline phosphatase liver/bone/kidney antibody, Alkaline phosphatase placental antibody, Alkaline phosphatase placental like 2 antibody, Alkaline phosphatase placental like antibody, Alkaline phosphatase placental type antibody, Alkaline phosphomonoesterase antibody, ALP 1 antibody, ALP antibody, ALP I antibody, ALP L antibody, ALP P antibody, ALPG antibody, ALPI antibody, ALPL antibody, ALPP antibody, ALPPL antibody, ALPPL2 antibody, AP TNAP antibody, FLJ40094 antibody, GCAP antibody, Germ cell alkaline phosphatase antibody, Glycerophosphatase antibody, HOPS antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody, Nagao isozyme antibody, OTTHUMP00000164357 antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP antibody, PLAP like antibody, Regan isozyme antibody, Testicular and thymus alkaline phosphatase antibody, Tissue non specific alkaline phosphatase antibody, Tissue nonspecific ALP antibody, TNAP antibody, TNSALP antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase(31-45aa DQAQETLKYALELQK), different from the related mouse and rat sequences by three amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.