ALKBH8 Polyclonal Antibody

Background:
ALKBH8 (alkylated DNA repair protein AlkB homolog 8) is a 664 amino acid protein that is encoded by a gene located on chromosome 11. ALKBH8 contains one RRM (RNA recognition motif) domain and belongs to the AlkB family of proteins. ALKBH8 is one of many homologs of the Escherichia coli protein AlkB. AlkB functions to protect DNA and RNA against damage from environmental methylating compounds by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzyme acts by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate and molecular oxygen as the oxidizing agent. Three isoforms exist for ALKBH8 due alternative splicing of the gene.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
4930562C03Rik, 8030431D03Rik, 9430088N01Rik, ABH8, alkB alkylation repair homolog 8 (E. coli), alkB alkylation repair homolog 8, AlkB homologue 8, ALKB8, ALKBH8, Alkylated DNA repair protein alkB homolog 8, FLJ38204, MGC10235, OTTHUMP00000211286, OTTHUMP00000211287, Probable alpha-ketoglutarate-dependent dioxygenase ABH8, RGD1304687, S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8, tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8

Immunogen: Full length fusion protein

Swissprot: Q96BT7
Gene Accession: BC015183

Calculated MW: 75 kDa
Observed MW: Refer to figures

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 0.6 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:25-1:100, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.