Alpha-1 foetoprotein polyclonal FITC polyclonal, anti-human

Quantity: 200 µl

Background:
The reactivity of the antiserum is restricted to alpha-foetoprotein. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against amniotic fluid, hepatoma sera and purified alpha-foetoprotein a single precipitin line is obtained. No precipitation reaction is obtained with normal human serum. To identify and measure alpha foetoprotein at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Isotype: Rabbit Polyclonal

Source: Purified human alpha-foetoprotein is isolated from pooled amniotic fluid and hepatoma serum, using a proprietary multiple step method. Several foetoproteins have been demonstrated in human foetal serum and amniotic fluid. One of the best studied is the alpha-foetoprotein. It cab easily be identified immunochemically because of its distinct antigenicity. In electrophoresis its relative mobility is between the albumin and alpha-1 globulin fractions. Its molecular weight is 64,000, the carbohydrate content is relatively low. The concentration of alpha-foetoprotein gradually decreases during foetal growth. It is not found in normal adult serum, but reappears in most human developing hepatoma. In pooled foetal/newborn serum a concentration of 1 mg/ml has been measured. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: FITC-coupled purified hyperimmune rabbit IgG, in phosphate buffered saline (PBS, pH 7.2).

Reactivity: human

Cross Reactivity:
The antiserum does not cross react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence

Storage: Store at 4C, or in small aliquots at -20C.