Alpha-1 orosomucoid polyclonal, anti-human

Quantity: 200 µl

Background:
The reactivity of the antiserum is restricted to alpha-foetoprotein. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against amniotic fluid, hepatoma sera and purified alpha-foetoprotein a single precipitin line is obtained. No precipitation reaction is obtained with normal human serum. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of alpha-1 orosomucoid or to determine its concentration.

Isotype: Rabbit Polyclonal

Source: Alpha-1 orosomucoid is a glycoprotein with a molecular weight of 41,000. It is an acute phase protein with a carbohydrate content of 42%. The mean concentration of this protein in serum is 900 ?g/ml. Concentrations are elevated in cases of tissue damage, inflammation, oestrogen administration and the presence of myeloma. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: human

Cross Reactivity:
The antiserum does not cross-react with any other Human plasma proteins as tested in geldiffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.