Alpha-2 macroglobulin polyclonal, anti-mouse

Quantity: 200 µl

Background:
The reactivity of the antiserum is restricted to alpha-2 macroglobulin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified alpha-2 macroglobulin. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence alpha-2 macroglobulin in mouse serum or other body fluids and to determine its concentration. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays, but this does not exclude such use if proper controls are included.

Isotype: Rabbit Polyclonal

Source: Alpha-2 macroglobulin ids the major protein in the alpha-2 region of mouse serum after separation by electrophoresis. It is a large polymer and a strong antigen. It forms about one-third in the total of the alpha-2 globulins. The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gel filtration and preparative starch block electrophoresis. In the purification of alpha-2 macroglobulin the activation of kallikrein is avoided, since it forms an irreversible bond with aplpha-2 macroglobulin, thus contaminating the final immunogen. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: mouse

Cross Reactivity:
Inter-species cross reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion (Ouchterlony) with the following results: Bovine ﾱ ferret ﾱ Horse ﾱ Rat ++ Cat ﾱ Goat - Human -Sheep - Chicken - Guinea Pig ﾱ Monkey - Swine ﾱ Dog - Hamster + pigeon - Turkey -

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.