alpha T-Catenin (clone 892_24D2S), anti-human

Background:
Catenins are proteins that connect cadherin/beta-Catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, alphaT-Catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed Human alphaT-Catenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that alphaT-Catenin can functionally restore cell-cell adhesion in colon cancer cells lacking alpha-Catenins. This indiCates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other alpha-Catenins to which it shows an overall amino acid identity of 57%. In cells transfected with alphaT-Catenin cDNA, interaction with beta-Catenin was demonstRated by co-immunoprecipitation. The 892_24D2S antibody anti alphaT-Catenin does not show any cross reaction with alphaE-Catenin or alphaN-Catenin in western blot analysis.

Clone: 892_24D2S

Isotype: Mouse IgG2a

Source: 892_24D2S is a Mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a C57Bl/6 Mouse immunized with a synthetic peptide corresponding to amino acids 164-177 of the Human alphaT-Catenin protein including an additional N-terminal cysteine (CVANKSDLQKTYQKL) coupled to keyhole limpet hemocyanin.

Reactivity: human

Specificity: 892_24D2S reacts with an epitope loCated between residues 164-177 of the Human alphaT-Catenin protein.

Form: Each vial contains 100 ul of 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide.

Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western blotting

Storage: Store at 4C, or in small aliquots at -20C.