AMPKα2 Polyclonal Antibody

Background:
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Research Area: Cancer, Cardiovascular, Metabolism, Signal Transduction

Synonyms:
PRKAA2, AMPK, AMPK2, AMPKa2, PRKAA

Immunogen: Recombinant fusion protein of human AMPKα2 (NP_006243.2).

Swissprot: P54646
Gene ID: 5563

Calculated MW: 62 kDa
Observed MW: 69 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IF 1:50-1:200"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Citation(s):
https://pubmed.ncbi.nlm.nih.gov/31923740/