Annexin A1 (clone M021), anti-human

Background:
The Annexin family is composed of at least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. Annexins have roles in membrane fusion, endocytosis, secretion, and repair. Annexin A1 binds to cellular membranes in a calcium-dependent manner, promotes membrane fusion and endocytosis, and has been implicated as an anti-inflammatory mediator. Annexin A2 is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A5 is a PKC inhibitor, directly interacts with VEGFR2 receptor, and binds phosphatidylserine to inhibit blood coagulation. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.

Background References
Grewal T & Enrich C (2009) Cell Signal. 21(6):847.
Hoque M et al. (2014) Cell Signal. 26(6):1213.
Boye TL & Nylandsted J (2016) Biol Chem. 397(10):961.

Immunogen: Clone M021 was generated from a proprietary antigen related to human Annexin A1 in the A549 lung carcinoma cell line.

Specificity: Clone M021 detects a 38 kDa* protein corresponding to the molecular mass of Annexin A1 on SDS-PAGE immunoblots of human A431, MDA-MB-231, and A549. The antibody also detects full length recombinant human Annexin A1, but not Annexin A2 or Annexin A5 recombinant proteins. The antibody works for western blot, ELISA, immunocytochemical labeling, and immunoprecipitation.

Application dilution:
ELISA: 1:1000
ICC: 1:100
IP: 1:100
WB: 1:1000
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Buffer/Storage:
Mouse monoclonal, protein G purified antibody is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.