Annexin A2 (C-terminal region) (clone M298), anti-human, mouse, rat, chicken
€441.00
In stock
SKU
ECM-AM2981
Catalog Number: ECM-AM2981
Size: 100 μl
Isotype: mouse IgG1
Applications: WB, E, IP, ICC, IHC
Reactivity: Hu, Ms, Rt, Ck
Datasheet
Questions? Contact us!
Size: 100 μl
Isotype: mouse IgG1
Applications: WB, E, IP, ICC, IHC
Reactivity: Hu, Ms, Rt, Ck
Datasheet
Questions? Contact us!
Background:
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin α1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. Annexin A2 (calpactin I, chromobindin 8, p36, Lipocortin II, PAP-IV, or Protein I) is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of cortocosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.
Immunogen: Clone (M298) was generated from a recombinant sequence that included amino acids in the C-terminal region of rat Annexin A2.
Specificity: This antibody detects a 36 kDa* protein corresponding to the molecular mass of Annexin A2 on SDS-PAGE immunoblots of rat PC12 cells. The antibody has been shown to label Annexin A2 in immunocytochemical analysis of PC12 cells and immunohistochemical analysis of mouse diaphragm.
Application dilution:
ELISA: 1:2000
ICC: 1:50
IHC: 1:50
IP: 1:50
WB: 1:1000
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Product References:
Moylan, J. et al. (2014) Redox biology 2:910. WB: C2C12 myoblast
Gilliam, L.A. et al. (2011) AJP Lung Cell Mol Phys 300:L225. IHC: mouse diaphragm
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin α1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. Annexin A2 (calpactin I, chromobindin 8, p36, Lipocortin II, PAP-IV, or Protein I) is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of cortocosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.
Immunogen: Clone (M298) was generated from a recombinant sequence that included amino acids in the C-terminal region of rat Annexin A2.
Specificity: This antibody detects a 36 kDa* protein corresponding to the molecular mass of Annexin A2 on SDS-PAGE immunoblots of rat PC12 cells. The antibody has been shown to label Annexin A2 in immunocytochemical analysis of PC12 cells and immunohistochemical analysis of mouse diaphragm.
Application dilution:
ELISA: 1:2000
ICC: 1:50
IHC: 1:50
IP: 1:50
WB: 1:1000
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Product References:
Moylan, J. et al. (2014) Redox biology 2:910. WB: C2C12 myoblast
Gilliam, L.A. et al. (2011) AJP Lung Cell Mol Phys 300:L225. IHC: mouse diaphragm
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