Annexin A2 (clone M009), anti-human

Background:
The Annexin family of is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. Annexins have roles in membrane fusion, endocytosis, secretion, and repair. Annexin A1 binds to cellular membranes in a calcium-dependent manner, promotes membrane fusion and endocytosis, and has been implicated as an anti-inflammatory mediator. Annexin A2 is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A5 is a PKC inhibitor, directly interacts with VEGFR2 receptor, and binds phosphatidylserine to inhibit blood coagulation. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.

Background References
Feinberg, J.M. et al. (1991) J Histochem & Cytochem. 39:955.
Grewal, T. & Enrich, C. (2006) Bioessays 28(12):1211.

Immunogen: Clone M009 was generated from a proprietary antigen related to human Annexin A2 in MDA-MB-231 breast cancer cell line.

Specificity: Clone M009 detects a 36 kDa* protein corresponding to the molecular mass of Annexin A2 on SDS-PAGE immunoblots of human A431, MDA-MB-231, MeWo, HUVEC, and PC3 cells. The antibody also works for ELISA, immunocytochemical labeling, and immunoprecipitation applications. In addition, mass spectrometry analysis of immunoprecipitates using AM0091 in human A431 cell lysates confirms that this antibody only detects Annexin A2 protein.

Application dilution:
ELISA: 1:1000
ICC: 1:100
IP-MS: 1:100
WB: 1:1000
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Buffer/Storage:
Mouse monoclonal, protein G purified antibody is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.