Apaf-1 polyclonal, anti-human, mouse

Background:
The mammalian homologs of the Ced-4 proteins, Apaf-1 (Ced-4), Nod1 (CARD4), and Nod2 contain a caspase recruitment domain (CARD) and a putative nucleotide binding domain, signified by a consensus Walker's A box (P-loop) and B box (Mg²⁺-binding site). Nod1 contains a putative regulatory domain and multiple leucine-rich repeats. Nod1 is a member of a growing family of intracellular proteins which share structural homology to the apoptosis regulator Apaf-1. Nod1 associates with the CARD-containing kinase RICK and activates NFkB. The self-association of Nod1 mediates proximity of RICK and the interaction of RICK with IKKg. In addition, Nod-1 binds to multiple caspases with long prodomains, but specifically activates caspase-9 and promotes caspase-9-induced apoptosis. Nod2 is composed of two N-terminal CARDs, a nucleotide-binding domain, and multiple C-terminal leucine-rich repeats. The expression of Nod2 is highly restricted to monocytes, and activates NFkB in response to bacterial lipopoly-saccharides.

Alternative Name:
APAF-1, APAF1, APAF 1, Apaf-1, APAF, Apaf1, Apoptotic peptidase activating factor 1, Apoptotic protease activating factor 1, Apoptotic protease activating factor, Apoptotic protease-activating factor 1, CED 4, CED4, KIAA0413,

Application Dilution:
WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: Apaf-1 polyclonal antibody detects endogenous levels of Apaf-1 protein.

Immunogen:
recombinant protein

MW: 141 kDa

Swiss-Prot: O14727(Human) O88879(Mouse)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.