APC (C-terminal region) (clone KT45), anti-human, mouse, rat

Background:
The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and MT association with organelles by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. One group of +TIPs include proteins with basic and serine-rich motifs (SxIP motifs) that mediate interaction between MTs and EB proteins. Adenomatous polyposis coli (APC), MACF, and STM1 are a group of the SxIP motif-containing proteins. APC protein is a large multidomain tumor suppresor protein that has important roles in Wnt signaling, as well as several other cell functions including cell migration, spindle assembly, chromosome segregation, neuronal differentiation, apoptosis, and MT stabilization. APC interaction with EB proteins through its SxIP motif promotes interaction with MTs leading to stabilization and increased polymerization.

Immunogen: Clone KT45 was generated from a recombinant protein containing amino acid residues 2170 to 2394 from mouse APC. This sequence is highly conserved in human and rat APC, and has low homology to APC2.

Specificity: This antibody detects a primarily full length APC at 300 kDa* in human Hct116 cells, mouse brain, and in rat PC12 cells. In immunocytochemistry, the antibody detects in APC in clusters at the plasma membrane and at the ends of microtubules.

Application dilution:
ELISA: 1:1000
ICC: 1:50
WB: 1:250
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Buffer/Storage:
Rat monoclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.