APE1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
APEX1, also called apurinic endonuclease(APE), is a DNA repair enzyme having apurinic/apyrimidinic(AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.

Description:
Rabbit IgG polyclonal antibody for DNA- (apurinic or apyrimidinic site) lyase (APEX1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
AP endonuclease 1 antibody, AP endonuclease class I antibody, AP lyase antibody, APE 1 antibody, APE antibody, APE-1 antibody, APEN antibody, APEX 1 antibody, APEX antibody, APEX nuclease(multifunctional DNA repair enzyme) 1 antibody, Apex nuclease 1 antibody, APEX nuclease antibody, APEX1 antibody, APEX1_HUMAN antibody, Apurinic endonuclease antibody, Apurinic-apyrimidinic endonuclease 1 antibody, Apurinic/apyrimidinic(abasic) endonuclease antibody, Apurinic/apyrimidinic endonuclease 1 antibody, Apurinic/apyrimidinic exonuclease antibody, APX antibody, BAP1 antibody, Deoxyribonuclease(apurinic or apyrimidinic) antibody, DNA(apurinic or apyrimidinic site) lyase antibody, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody, EC 4.2.99.18 antibody, HAP 1 antibody, HAP1 antibody, Human Apurinic endonuclease 1 antibody, MGC139790 antibody, Multifunctional DNA repair enzyme antibody, Redox factor 1 antibody, Redox factor-1 antibody, REF 1 antibody, REF 1 protein antibody, REF-1 antibody, REF1 antibody, REF1 protein antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human APE1(177-191aa RGLVRLEYRQRWDEA), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.