Apg12 (Atg12) monoclonal, anti-human

Description: Anti-Apg12 (Atg12) Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Protein Function:
Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus..

Other Names: Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like, ATG12 (HGNC:588), APG12, APG12L

Clone Name(s):
Clone: ABID-1

Subcellular Localization: Cytoplasm. Preautophagosomal structure membrane ; Peripheral membrane protein. TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.

Tissue Specificity: Ubiquitous..

Gene ID: 9140

Immunogen: A synthesized peptide derived from human Apg12 (Atg12)

Calculated MW: 15113

Purification: Affinity-chromatography

Format: Liquid

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Concentration (mg/ml): Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.