APG5L/ATG5 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is also known as APG5 or ASP, and this gene is mapped to 6q21. It is found that knockdown of ATG5 in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation. These hepatocytes with ATG5 knockdown also had increased lipid droplet number and size. ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.

Description:
Rabbit IgG polyclonal antibody for Autophagy protein 5 (ATG5) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
APG 5 antibody, APG 5L antibody, APG5 antibody, APG5 autophagy 5 like antibody, APG5 like antibody, APG5-like antibody, APG5L antibody, Apoptosis specific protein antibody, Apoptosis-specific protein antibody, ASP antibody, ATG 5 antibody, Atg5 antibody, ATG5 autophagy related 5 homolog antibody, Autophagy protein 5 antibody, hAPG5 antibody, Homolog of S Cerevisiae autophagy 5 antibody, OTTHUMP00000040507 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human APG5L(82-97aa DRFDQFWAINRKLMEY), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.