Apoptosis repressor with CARD polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
NOL3(Nucleolar protein 3), also known as ARC, NOP30, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al.(1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the 2 isoforms is located in exon 2. It is reported that expression of the ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. ARC also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and CASP8 through its N-terminal death effector domain but does not interact with CASP1, CASP3, or CASP9.

Description:
Rabbit IgG polyclonal antibody for Nucleolar protein 3 (NOL3) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
Apoptosis repressor with CARD antibody, ARC antibody, Muscle enriched cytoplasmic protein antibody, Muscle-enriched cytoplasmic protein antibody, MYC antibody, MYP antibody, Nol3 antibody, NOL3_HUMAN antibody, NOP antibody, Nop30 antibody, Nucleolar protein 3(apoptosis repressor with CARD domain) antibody, Nucleolar protein 3 antibody, Nucleolar protein of 30 kDa antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD(91-106aa WDWQHVGPGYRDRSYD), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD(91-106aa WDWQHVGPGYRDRSYD), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.