APRIL polyclonal, anti-human
€0.00
In stock
SKU
AC-ABG10012
Catalog Number: AC-ABG10012
Size(s): 50 µg, 100 µg
Host: Rabbit
Applications: WB, IHC, E
Datasheet
Request Information
Size(s): 50 µg, 100 µg
Host: Rabbit
Applications: WB, IHC, E
Datasheet
Request Information
Source:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hAPRIL. Anti-Human APRIL specific antibody was purified by affinity chromatography employing immobilized hAPRIL matrix.
Western Blot:
To detect hAPRIL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAPRIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect hAPRIL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human APRIL (60-066BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hAPRIL.
Immunohistochemistry:
This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 0.25 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hAPRIL. Anti-Human APRIL specific antibody was purified by affinity chromatography employing immobilized hAPRIL matrix.
Western Blot:
To detect hAPRIL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAPRIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect hAPRIL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human APRIL (60-066BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hAPRIL.
Immunohistochemistry:
This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 0.25 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
| Is Featured? | No |
|---|
Write Your Own Review