Aquaporin 3 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
AQP3, Aquaporin 3, is the third member of the major intrinsic protein family from rat kidney. AQP3 is localized at the basal lateral membranes of collecting duct cells in the kidney. AQP3 appears to exist as a single copy and to comprise 6 exons distributed over 7 kb. The AQP3 gene is mapped to 9p21-p12 by fluorescence in situ hybridization. AQP3 works as water-channel. And it facilitates the transport of nonionic small solutes such as urea and glycerol, albeit to a smaller degree. The results suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms.By Western blot analysis and immunofluorescence microscopy of epidermis, Sougrat et al.(2002) showed strong expression of an approximately 33-kD glycosylated AQP3 protein in keratinocyte plasma membranes, 1 layer below the unstained stratum corneum(SC). AQP3 provides a short circuit for water, or water-clamp, between the base of the epidermis and the SC in order to maintain a constant water content and to prevent the formation of a continuous water gradient across the epidermis below the SC.

Description:
Rabbit IgG polyclonal antibody for Aquaporin-3 (AQP3) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.

Synonyms:
AQP 3 antibody, AQP-3 antibody, Aqp3 antibody, AQP3_HUMAN antibody, Aquaglyceroporin-3 antibody, Aquaporin 3(GIL blood group) antibody, Aquaporin 3(Gill blood group) antibody, Aquaporin-3 antibody, Aquaporin3 antibody, GIL antibody, Gill blood group antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 3(278-292aa EENVKLAHVKHKEQI), different from the related rat and mouse sequences by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, IHC-F, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.