Arachidonic acid (AA) detection kit

Use: Used to detect the concentration of arachidonic acid (AA) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled arachidonic acid (AA) is used, and the purified anti-arachidonic acid (AA) antibody is coated on the microplate. In the competitive inhibition reaction, a certain amount of solid-phase antibody inhibits the competitive reaction with biotin-labeled arachidonic acid (AA) and non-labeled antigen (calibrator or specimen). The binding amount of antibody and biotin-labeled arachidonic acid (AA) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of antibody and biotin-labeled arachidonic acid (AA), and vice versa. After the reaction is balanced, solid-phase antibody-biotinylated arachidonic acid (AA) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated arachidonic acid (AA)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of arachidonic acid (AA) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of arachidonic acid (AA) in samples.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.