ARF-BP1 polyclonal, anti-human, mouse, rat

Protein Family: Enzymes

Pathway and Disease: Signaling Molecules and Interaction

Description:
The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.

Alternate Names: ARF-BP1, ARF binding protein 1, HECT, UBA and WWE domain containing 1, HUWE1, Mcl-1 ubiquitin ligase E3, Mule

Application Notes: E: 1:500-1:1,000; WB: 1:100-1:500; ICC: 1:100-1:500

Accession No.: NP_113584

Antigen: KLH-conjugated synthetic peptide encompassing a sequence within human ARF-BP1.

Format: Each vial contains 0.1 ml IgG in PBS pH 7.4 with 0.02% sodium azide. Antibody was purified by immunogen affinity chromatography.

Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.