ARF1 polyclonal, anti-human, mouse, rat

Background:
ADP-ribosylation factors (ARFs), are small guanine nucleotide-binding proteins that enhance the enzymatic activities of cholera toxin, and constitute one family of the RAS superfamily. ARFs are essential and ubiquitous in eukaryotes, as they are involved in vesicular transport and functioning via phospholipase D activation. ARF proteins play a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids. ARF1 is regulated by the binding and hydrolysis of GTP. Coatomer, or COPI, is a heptameric protein recruited to membranes by ARF1. Research demonstrates that guanine nucleotide exchange-activated ARF1, when located at the Golgi membrane, recruits and binds cytoplasmic COPI to the membranes.

Alternative Name:
ARF1, ADP Ribosylation Factor 1, ADP-ribosylation factor 1, ARF 1, ARF1, ARF1,

Application Dilution:
WB:1:1,000-1:2,000
IHC:1:50-1:200
FC:1:50-1:100

Specificity: ARF1 polyclonal antibody detects endogenous levels of ARF1 protein.

Immunogen:
recombinant protein

MW: 21 kDa

Swiss-Prot: P84077(Human) P84078(Mouse) P84079(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.