ARF6 monoclonal, anti-human, mouse, rat

Description: Anti-ARF6 Rabbit Monoclonal Antibody . Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Protein Function:
GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase..

Other Names: ADP-ribosylation factor 6 {ECO:0000303|Ref.6}, 3.6.5.2, ARF6 {ECO:0000303|Ref.6, ECO:0000312|HGNC:HGNC:659}

Clone Name(s):
Clone: AOFF-1

Subcellular Localization: Golgi apparatus. Cell membrane; Lipid- anchor. Endosome membrane; Lipid-anchor. Recycling endosome membrane ; Lipid-anchor. Cell projection, filopodium membrane; Lipid-anchor. Midbody. Cytoplasm. Cleavage furrow. Distributed throughout the cytoplasm during metaphase. Transiently detected at the ingressing cleavage furrow during mitotic cytokinesis. Recruited to the midbody at later stages of cytokinesis; this requires interaction with KIF23 (By similarity). Recruited to the cell membrane in association with CYTH2 and ARL4C. Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles..

Tissue Specificity: Ubiquitous, with higher levels in heart, substantia nigra, and kidney..

Gene ID: 382

Immunogen: A synthesized peptide derived from human ARF6

Calculated MW: 20082

Purification: Affinity-chromatography

Format: Liquid

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Concentration (mg/ml): Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.