Argonaute 2 (phospho-Tyr-393) Blocking Peptide
€155.00
In stock
SKU
ECM-AX5315
Background:
Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response. These small RNAs function together with complexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation or stability. Among the most important components in these complexes are argonaute proteins. There are four members in the mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonaute proteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFR phosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylates Ago2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression.
Sequence: Phospho-Ago2 (Tyr-393) synthetic peptide corresponding to amino acids surrounding tyrosine 393 in human Ago2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2.
Specificity: The peptide is specifically recognized by anti-Argonaute 2 (Tyr-393) phospho-specific antibody (AP5311) in ELISA, and has been shown to block the reactivity of AP5311 in Western blot. In addition, the peptide is recommended for use in blocking AP5311 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response. These small RNAs function together with complexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation or stability. Among the most important components in these complexes are argonaute proteins. There are four members in the mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonaute proteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFR phosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylates Ago2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression.
Sequence: Phospho-Ago2 (Tyr-393) synthetic peptide corresponding to amino acids surrounding tyrosine 393 in human Ago2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2.
Specificity: The peptide is specifically recognized by anti-Argonaute 2 (Tyr-393) phospho-specific antibody (AP5311) in ELISA, and has been shown to block the reactivity of AP5311 in Western blot. In addition, the peptide is recommended for use in blocking AP5311 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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